Hotspot

trnL-CAA-ccsA

SSC · 113129-113220 · 92 bp

Back to Periandra

Pi 0.093

MarkerSeek score 75.60

Primer available yes

Label trnL-CAA-ccsA

Feature Metadata

label_name: trnL-CAA-ccsA
feature_type: igs
region: SSC
length_bp: 92
variable_sites: 8
pi: 0.093
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ccsA_p1	AAATGGTAGACACGCGAGAC	AAAAAGGCCCGAAGTAGCAA	50.0	45.0	58.0	58.0	521 bp	0.5	59.9
trnL-CAA-ccsA_p2	AAATGGTAGACACGCGAGAC	AAAAGGCCCGAAGTAGCAAA	50.0	45.0	58.0	58.0	520 bp	0.5	59.9
trnL-CAA-ccsA_p3	TGAAATGGTAGACACGCGAG	AAAAAGGCCCGAAGTAGCAA	50.0	45.0	58.0	58.0	523 bp	0.5	59.8
trnL-CAA-ccsA_p4	TGAAATGGTAGACACGCGAG	AAAAGGCCCGAAGTAGCAAA	50.0	45.0	58.0	58.0	522 bp	0.5	59.8
trnL-CAA-ccsA_p5	GCCTTGGTGGTGAAATGGTA	AAAAGGCCCGAAGTAGCAAA	50.0	45.0	58.1	58.0	532 bp	0.5	59.3