Hotspot

rpl32-trnL-CAA

SSC · 112521-113047 · 527 bp

Back to Periandra

Pi 0.0368
MarkerSeek score 67.50
Primer available yes
Label rpl32-trnL-CAA

Feature Metadata

label_name
rpl32-trnL-CAA
feature_type
igs
region
SSC
length_bp
527
variable_sites
18
pi
0.0368
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-CAA_p1 TCCTAATTGGATGGCAGTTCC AAAAAGGCCCGAAGTAGCAA 47.6 45.0 57.7 58.0 1192-1232 bp 1.0 76.2
rpl32-trnL-CAA_p2 TCCTAATTGGATGGCAGTTCC AAAAGGCCCGAAGTAGCAAA 47.6 45.0 57.7 58.0 1191-1231 bp 1.0 76.2
rpl32-trnL-CAA_p3 TCCTAATTGGATGGCAGTTCC GTCTCGCGTGTCTACCATTT 47.6 50.0 57.7 58.0 731 bp 0.5 49.6
rpl32-trnL-CAA_p4 TCCTAATTGGATGGCAGTTCC CTCGCGTGTCTACCATTTCA 47.6 50.0 57.7 58.0 729 bp 0.5 49.5
rpl32-trnL-CAA_p5 TCCTAATTGGATGGCAGTTCC TACCATTTCACCACCAAGGC 47.6 50.0 57.7 58.1 719 bp 0.5 49.0