Hotspot

ndhF-rpl32

SSC · 111649-112358 · 710 bp

Back to Periandra

Pi 0.0272

MarkerSeek score 62.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 710
variable_sites: 19
pi: 0.0272
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCCCACATACGACGAAGATT	GTCTCGCGTGTCTACCATTT	50.0	50.0	58.1	58.0	1553 bp	0.5	59.4
ndhF-rpl32_p2	GCCCACATACGACGAAGATT	CTCGCGTGTCTACCATTTCA	50.0	50.0	58.1	58.0	1551 bp	0.5	59.4
ndhF-rpl32_p3	AGGAAAAGCCCACATACGAC	GTCTCGCGTGTCTACCATTT	50.0	50.0	57.9	58.0	1560 bp	0.5	59.1
ndhF-rpl32_p4	AGGAAAAGCCCACATACGAC	CTCGCGTGTCTACCATTTCA	50.0	50.0	57.9	58.0	1558 bp	0.5	59.1
ndhF-rpl32_p5	GCCCACATACGACGAAGATT	TACCATTTCACCACCAAGGC	50.0	50.0	58.1	58.1	1541 bp	0.5	58.8