Hotspot

rps16-accD

LSC · 56869-57509 · 641 bp

Back to Periandra

Pi 0.0159

MarkerSeek score 69.20

Primer available yes

Label rps16-accD

Feature Metadata

label_name: rps16-accD
feature_type: igs
region: LSC
length_bp: 641
variable_sites: 9
pi: 0.0159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-accD_p1	AGATCTTCGGAAAGAGGGCT	TCCACACCTGTATTCTAACTTCC	50.0	43.5	58.1	57.6	682-756 bp	1.0	56.7
rps16-accD_p2	AGATCTTCGGAAAGAGGGCT	CCACACCTGTATTCTAACTTCCT	50.0	43.5	58.1	57.6	681-755 bp	1.0	56.6
rps16-accD_p3	GAGATCTTCGGAAAGAGGGC	TCCACACCTGTATTCTAACTTCC	55.0	43.5	57.8	57.6	683-757 bp	1.0	55.8
rps16-accD_p4	GAGATCTTCGGAAAGAGGGC	CCACACCTGTATTCTAACTTCCT	55.0	43.5	57.8	57.6	682-756 bp	1.0	55.8
rps16-accD_p5	GGAATTGATGTTCGATCGCG	TCCACACCTGTATTCTAACTTCC	50.0	43.5	57.7	57.6	714-788 bp	1.0	55.3