Hotspot

atpA-trnR-UCU

LSC · 51857-52252 · 396 bp

Back to Periandra

Pi 0.0319
MarkerSeek score 68.10
Primer available yes
Label atpA-trnR-UCU

Feature Metadata

label_name
atpA-trnR-UCU
feature_type
igs
region
LSC
length_bp
396
variable_sites
12
pi
0.0319
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA-trnR-UCU_p1 TGAAGGAAGCTATTCAGGAACA AAGAGAAGCGTCCATTGTCT 40.9 45.0 57.1 56.9 521-540 bp 1.0 52.4
atpA-trnR-UCU_p2 TGAAGGAAGCTATTCAGGAACA AAAGAGAAGCGTCCATTGTCT 40.9 42.9 57.1 57.6 522-541 bp 1.0 50.3
atpA-trnR-UCU_p3 TGAAGGAAGCTATTCAGGAACAA AAGAGAAGCGTCCATTGTCT 39.1 45.0 57.8 56.9 521-540 bp 1.0 49.8
atpA-trnR-UCU_p4 TTGAAGGAAGCTATTCAGGAACA AAGAGAAGCGTCCATTGTCT 39.1 45.0 57.8 56.9 522-541 bp 1.0 49.8
atpA-trnR-UCU_p5 GGAAGCTATTCAGGAACAAATGG AAGAGAAGCGTCCATTGTCT 43.5 45.0 57.7 56.9 517-536 bp 1.0 49.7