Hotspot

psbD-trnT-GGU

LSC · 28490-29898 · 1409 bp

Back to Periandra

Pi 0.0193

MarkerSeek score 70.70

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1409
variable_sites: 27
pi: 0.0193
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	AAGCAATAGACCGGACCAAC	GTGGTAGAGTAACGCCATGG	50.0	55.0	57.9	58.1	1580-1581 bp	1.0	80.6
psbD-trnT-GGU_p2	AAGCAATAGACCGGACCAAC	TCAGTGGTAGAGTAACGCCA	50.0	50.0	57.9	58.1	1583-1584 bp	1.0	80.5
psbD-trnT-GGU_p3	CACAAAAACGAAACGGTCCC	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.2	58.1	1559-1560 bp	1.0	80.2
psbD-trnT-GGU_p4	CCACAAAAACGAAACGGTCC	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.2	58.1	1560-1561 bp	1.0	80.2
psbD-trnT-GGU_p5	CCCACAAAAACGAAACGGTC	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.2	58.1	1561-1562 bp	1.0	80.2