Hotspot

trnT-UGU-rps4

LSC · 15115-15395 · 281 bp

Back to Periandra

Pi 0.036

MarkerSeek score 66.70

Primer available yes

Label trnT-UGU-rps4

Feature Metadata

label_name: trnT-UGU-rps4
feature_type: igs
region: LSC
length_bp: 281
variable_sites: 10
pi: 0.036
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-rps4_p1	CTCAGAGGTTAGAGCATCGC	AGGTCCTCTGTAACGTGACA	55.0	50.0	57.9	58.0	362-365 bp	1.0	81.7
trnT-UGU-rps4_p2	GGTTCGATTCCGATAGCTGG	AGGTCCTCTGTAACGTGACA	55.0	50.0	58.2	58.0	321-324 bp	1.0	81.4
trnT-UGU-rps4_p3	CTCAGAGGTTAGAGCATCGC	TCGCTTTTTACTGTTGGCCT	55.0	45.0	57.9	58.0	433-436 bp	1.0	81.1
trnT-UGU-rps4_p4	ATGGTCATCGGTTCGATTCC	AGGTCCTCTGTAACGTGACA	50.0	50.0	57.8	58.0	330-333 bp	1.0	81.1
trnT-UGU-rps4_p5	GGTTCGATTCCGATAGCTGG	TCGCTTTTTACTGTTGGCCT	55.0	45.0	58.2	58.0	392-395 bp	1.0	80.7