Pi
0.0258
MarkerSeek score
72.00
Primer available
yes
Label
trnL-UAA-trnT-UGU
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnL-UAA-trnT-UGU_p1 | ACCAATTTCGCCATATCCCC | GCGATGCTCTAACCTCTGAG | 50.0 | 55.0 | 58.0 | 57.9 | 1078-1103 bp | 1.0 | 84.1 |
| trnL-UAA-trnT-UGU_p2 | ACCAATTTCGCCATATCCCC | AAAGCCAGCTATCGGAATCG | 50.0 | 50.0 | 58.0 | 58.1 | 1123-1148 bp | 1.0 | 83.9 |
| trnL-UAA-trnT-UGU_p3 | TAGCGTCTACCAATTTCGCC | GCGATGCTCTAACCTCTGAG | 50.0 | 55.0 | 58.1 | 57.9 | 1086-1111 bp | 1.0 | 83.6 |
| trnL-UAA-trnT-UGU_p4 | TAGCGTCTACCAATTTCGCC | AAAGCCAGCTATCGGAATCG | 50.0 | 50.0 | 58.1 | 58.1 | 1131-1156 bp | 1.0 | 83.4 |
| trnL-UAA-trnT-UGU_p5 | ACCAATTTCGCCATATCCCC | TCGAGAAAAGCCAGCTATCG | 50.0 | 50.0 | 58.0 | 57.8 | 1129-1154 bp | 1.0 | 83.3 |