Hotspot

ndhF

SSC · 111621-113864 · 2244 bp

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Pi 0.229

MarkerSeek score 55.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2244
variable_sites: 30
pi: 0.229
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.06
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GAGCTACTGAGGAACAACGG	AAGAACCGGCGAATCGTAAA	55.0	45.0	58.0	57.9	2646 bp	0.5	59.3
ndhF_p2	CCCGGAACTTCTTCGTAGTG	AAGAACCGGCGAATCGTAAA	55.0	45.0	58.0	57.9	2551 bp	0.5	59.3
ndhF_p3	CCGGAACTTCTTCGTAGTGG	AAGAACCGGCGAATCGTAAA	55.0	45.0	58.0	57.9	2550 bp	0.5	59.3
ndhF_p4	ACTCCCGGAACTTCTTCGTA	AAGAACCGGCGAATCGTAAA	50.0	45.0	58.1	57.9	2554 bp	0.5	58.7
ndhF_p5	TCCCGGAACTTCTTCGTAGT	AAGAACCGGCGAATCGTAAA	50.0	45.0	58.1	57.9	2552 bp	0.5	58.7