Hotspot

ndhF-rpl32

SSC · 113865-115099 · 1235 bp

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Pi 0.276

MarkerSeek score 75.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1235
variable_sites: 48
pi: 0.276
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.14
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGTCGTCGGAAAAAGGA	TTAACGCTGCCCAATATCCC	45.0	50.0	57.7	58.0	1422 bp	0.5	57.3
ndhF-rpl32_p2	TGTTGTCGTCGGAAAAAGGA	AAAAGCTTTTAACGCTGCCC	45.0	45.0	57.7	57.9	1430 bp	0.5	56.3
ndhF-rpl32_p3	CGGAAAAAGGAGAAGTCCCA	TTAACGCTGCCCAATATCCC	50.0	50.0	57.4	58.0	1413 bp	0.5	55.3
ndhF-rpl32_p4	CGGAAAAAGGAGAAGTCCCA	AAAAGCTTTTAACGCTGCCC	50.0	45.0	57.4	57.9	1421 bp	0.5	54.3
ndhF-rpl32_p5	TCGGAAAAAGGAGAAGTCCC	TTAACGCTGCCCAATATCCC	50.0	50.0	57.1	58.0	1414 bp	0.5	53.0