Hotspot

rps19-rpl2

IRb · 88334-88390 · 57 bp

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Pi 0.0702

MarkerSeek score 70.60

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 57
variable_sites: 4
pi: 0.0702
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	AAATGGGCAAGTGTTCCCTT	ATCCCAAAACTGCTCAGCAA	45.0	45.0	57.9	58.0	518-524 bp	1.0	80.6
rps19-rpl2_p2	AGTCGGTACAAACTCTCCCA	ATCCCAAAACTGCTCAGCAA	50.0	45.0	58.0	58.0	568 bp	0.5	60.0
rps19-rpl2_p3	AGTCGGTACAAACTCTCCCA	TGGGTAGAGCCGGATCTAAG	50.0	55.0	58.0	58.0	506 bp	0.5	59.9
rps19-rpl2_p4	AGTCGGTACAAACTCTCCCA	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.0	58.1	407 bp	0.5	59.5
rps19-rpl2_p5	AGTCGGTACAAACTCTCCCA	AAAAGTTTGGGTAGAGCCGG	50.0	50.0	58.0	58.1	513 bp	0.5	59.2