Hotspot

rpl33-rps18

LSC · 71849-72177 · 329 bp

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Pi 0.1
MarkerSeek score 74.90
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
329
variable_sites
33
pi
0.1
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 TCTGTCCCTATTGTTACAGACA AAGGGTCGCTTGGATTTATC 40.9 45.0 56.2 55.5 402-407 bp 1.0 46.5
rpl33-rps18_p2 TCTGTCCCTATTGTTACAGACA AAAGGGTCGCTTGGATTTATC 40.9 42.9 56.2 56.3 403-408 bp 1.0 46.5
rpl33-rps18_p3 TTCTGTCCCTATTGTTACAGACA AAGGGTCGCTTGGATTTATC 39.1 45.0 56.9 55.5 403-408 bp 1.0 46.5
rpl33-rps18_p4 TCTGTCCCTATTGTTACAGACAT AAGGGTCGCTTGGATTTATC 39.1 45.0 56.7 55.5 402-407 bp 1.0 46.5
rpl33-rps18_p5 TTCTGTCCCTATTGTTACAGACA AAAGGGTCGCTTGGATTTATC 39.1 42.9 56.9 56.3 404-409 bp 1.0 46.5