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trnV-UAC-accD

LSC · 59286-60682 · 1397 bp

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Pi 0.33

MarkerSeek score 80.80

Primer available yes

Label trnV-UAC-accD

Feature Metadata

label_name: trnV-UAC-accD
feature_type: igs
region: LSC
length_bp: 1397
variable_sites: 387
pi: 0.33
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-accD_p1	CCGATTCAGGTGCTCGTTTA	CCACCGATCCCATACTACCT	50.0	55.0	58.0	58.0	2962 bp	0.5	59.9
trnV-UAC-accD_p2	CCGATTCAGGTGCTCGTTTA	CCTACCACCGATCCCATACT	50.0	55.0	58.0	58.0	2966 bp	0.5	59.9
trnV-UAC-accD_p3	CCGATTCAGGTGCTCGTTTA	GCGGAATTTTGAGCAAGACC	50.0	50.0	58.0	58.0	2687 bp	0.5	59.8
trnV-UAC-accD_p4	CCGATTCAGGTGCTCGTTTA	GGCGGAATTTTGAGCAAGAC	50.0	50.0	58.0	58.0	2688 bp	0.5	59.8
trnV-UAC-accD_p5	CCGATTCAGGTGCTCGTTTA	GCGGCATATTCAATCAACCG	50.0	50.0	58.0	58.0	2999 bp	0.5	59.7