Hotspot

ycf1

IRb · 112955-114435 · 1481 bp

Back to Peperomia

Pi 0.0663

MarkerSeek score 69.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 1481
variable_sites: 67
pi: 0.0663
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.68
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TAGAGTTCAACCCCCGTTCT	CAATGGTGTTGGTGTTGCG	50.0	52.6	58.0	58.1	1936 bp	0.5	50.9
ycf1_p2	TGATGCACTGCCAGTACATC	CAATGGTGTTGGTGTTGCG	50.0	52.6	58.0	58.1	2283 bp	0.5	50.8
ycf1_p3	AATAGCCCCATCGGAGCTAT	CAATGGTGTTGGTGTTGCG	50.0	52.6	58.0	58.1	2233 bp	0.5	50.7
ycf1_p4	TCATAGAGTTCAACCCCCGT	CAATGGTGTTGGTGTTGCG	50.0	52.6	58.1	58.1	1939 bp	0.5	50.6
ycf1_p5	ACTCCACCTTTGATGCACTG	CAATGGTGTTGGTGTTGCG	50.0	52.6	58.1	58.1	2293 bp	0.5	50.3