Hotspot

ndhD

SSC · 119330-120826 · 1497 bp

Back to Peperomia

Pi 0.016

MarkerSeek score 53.30

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1497
variable_sites: 24
pi: 0.016
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CAACGCTTACGGGGTATGAA	GATCCAATGTCGCACTCAGT	50.0	50.0	58.0	58.0	2271-2282 bp	1.0	83.1
ndhD_p2	CAACGCTTACGGGGTATGAA	TGTGAATCTGCCTGTCCAAC	50.0	50.0	58.0	58.1	2106-2117 bp	1.0	82.6
ndhD_p3	CAACGCTTACGGGGTATGAA	TGATACCTTGGGACGGATGT	50.0	50.0	58.0	58.1	2183-2194 bp	1.0	82.3
ndhD_p4	CAACGCTTACGGGGTATGAA	GCTTGCCCCACAGATGTATT	50.0	50.0	58.0	58.2	2208-2219 bp	1.0	81.5
ndhD_p5	ATCAACGCTTACGGGGTATG	GATCCAATGTCGCACTCAGT	50.0	50.0	57.8	58.0	2273-2284 bp	1.0	81.3