Hotspot

rps15-ycf1

SSC · 127114-127612 · 499 bp

Back to Peperomia

Pi 0.174

MarkerSeek score 78.20

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 499
variable_sites: 70
pi: 0.174
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TACGACTCAGCTCCAGCATA	ATGTTTCGCGCATAATTGCC	50.0	45.0	57.9	58.2	1874 bp	0.5	58.3
rps15-ycf1_p2	TATACGACTCAGCTCCAGCA	ATGTTTCGCGCATAATTGCC	50.0	45.0	57.9	58.2	1876 bp	0.5	58.3
rps15-ycf1_p3	ATGAGTCGAAGAACACCGTG	ATGTTTCGCGCATAATTGCC	50.0	45.0	57.9	58.2	1629 bp	0.5	58.2
rps15-ycf1_p4	TGGTTCCCGGATATCCAGTT	ATGTTTCGCGCATAATTGCC	50.0	45.0	58.1	58.2	1420 bp	0.5	57.8
rps15-ycf1_p5	TTGGTTCCCGGATATCCAGT	ATGTTTCGCGCATAATTGCC	50.0	45.0	58.1	58.2	1421 bp	0.5	57.8