Hotspot

rpl23-trnI-CAU

IRb · 89364-89525 · 162 bp

Back to Peperomia

Pi 0.0688
MarkerSeek score 78.30
Primer available yes
Label rpl23-trnI-CAU

Feature Metadata

label_name
rpl23-trnI-CAU
feature_type
igs
region
IRb
length_bp
162
variable_sites
11
pi
0.0688
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23-trnI-CAU_p1 TCGACCCAATGCTTGATTTCT GAATGGTTAAAGCGCCCAAC 42.9 50.0 57.9 58.0 341-343 bp 1.0 74.4
rpl23-trnI-CAU_p2 TCGACCCAATGCTTGATTTCT CCTGTTAAGCATCCATGGCT 42.9 50.0 57.9 57.9 361-363 bp 1.0 73.8
rpl23-trnI-CAU_p3 CGACCCAATGCTTGATTTCTG GAATGGTTAAAGCGCCCAAC 47.6 50.0 57.9 58.0 340-342 bp 1.0 73.8
rpl23-trnI-CAU_p4 CGACCCAATGCTTGATTTCTG CCTGTTAAGCATCCATGGCT 47.6 50.0 57.9 57.9 360-362 bp 1.0 73.2
rpl23-trnI-CAU_p5 TCGACCCAATGCTTGATTTCT AATGGTTAAAGCGCCCAACT 42.9 45.0 57.9 58.4 340-342 bp 1.0 71.5