Hotspot

rps19-rpl2

IRb · 87438-87571 · 134 bp

Back to Peperomia

Pi 0.0687

MarkerSeek score 80.90

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 134
variable_sites: 9
pi: 0.0687
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	GCTCGGGACCAGGTTACTAT	TTATTCTTCGCCGCCGTAAA	55.0	45.0	58.6	58.0	267-269 bp	1.0	78.8
rps19-rpl2_p2	GCTCGGGACCAGGTTACTAT	TTTATTCTTCGCCGCCGTAA	55.0	45.0	58.6	58.0	268-270 bp	1.0	78.8
rps19-rpl2_p3	GCTCGGGACCAGGTTACTAT	TTTTATTCTTCGCCGCCGTA	55.0	45.0	58.6	58.0	269-271 bp	1.0	78.8
rps19-rpl2_p4	GCTCGGGACCAGGTTACTAT	GTTATCCTGCGCTTGGAAGA	55.0	50.0	58.6	58.0	321-323 bp	1.0	78.1
rps19-rpl2_p5	GCTCGGGACCAGGTTACTAT	GGGCCCCAATTGGTAGAAAA	55.0	50.0	58.6	58.1	360-362 bp	1.0	77.5