Hotspot

rps3-rpl22

LSC · 86585-86673 · 89 bp

Back to Peperomia

Pi 0.135
MarkerSeek score 77.40
Primer available yes
Label rps3-rpl22

Feature Metadata

label_name
rps3-rpl22
feature_type
igs
region
LSC
length_bp
89
variable_sites
10
pi
0.135
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.83
markerseek_score
77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3-rpl22_p1 AACCAAATGGAACGATGGCT TGAGTACTGCTACTTCACTTCC 45.0 45.5 58.1 57.2 168-183 bp 1.0 65.7
rps3-rpl22_p2 ACCAAATGGAACGATGGCTT TGAGTACTGCTACTTCACTTCC 45.0 45.5 58.1 57.2 167-182 bp 1.0 65.7
rps3-rpl22_p3 ACCAAGTCGTAAACCCAGTG TGAGTACTGCTACTTCACTTCC 50.0 45.5 57.8 57.2 139-154 bp 1.0 65.2
rps3-rpl22_p4 TGTGCAAACCAAATGGAACG TGAGTACTGCTACTTCACTTCC 45.0 45.5 57.8 57.2 174-189 bp 1.0 64.4
rps3-rpl22_p5 ACCAAATGGAACGATGGCTT GAGTACTGCTACTTCACTTCCT 45.0 45.5 58.1 56.9 166-181 bp 1.0 63.5