Hotspot

rps8-rpl14

LSC · 83560-83793 · 234 bp

Back to Peperomia

Pi 0.0526

MarkerSeek score 74.40

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 234
variable_sites: 12
pi: 0.0526
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	AGCAATAGTGTCCCTACCCA	AGGAACCCGAGTTTTTGGAG	50.0	50.0	57.7	57.7	343-344 bp	1.0	79.9
rps8-rpl14_p2	AGCAATAGTGTCCCTACCCA	CGCTCGGGAATTGAGACAAT	50.0	50.0	57.7	58.3	319-320 bp	1.0	79.6
rps8-rpl14_p3	AGCAATAGTGTCCCTACCCA	GATCGCTCGGGAATTGAGAC	50.0	55.0	57.7	58.5	322-323 bp	1.0	78.3
rps8-rpl14_p4	CAGCAATAGTGTCCCTACCC	AGGAACCCGAGTTTTTGGAG	55.0	50.0	57.4	57.7	344-345 bp	1.0	77.0
rps8-rpl14_p5	CAGCAATAGTGTCCCTACCC	CGCTCGGGAATTGAGACAAT	55.0	50.0	57.4	58.3	320-321 bp	1.0	76.8