Hotspot

trnP-UGG-psaJ

LSC · 69353-69820 · 468 bp

Back to Peperomia

Pi 0.0556

MarkerSeek score 79.80

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 468
variable_sites: 25
pi: 0.0556
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	AAAACAAACGCGCTACCAAG	TCTCTATCAACAGACCCGCT	45.0	50.0	57.9	57.9	588-604 bp	1.0	83.7
trnP-UGG-psaJ_p2	AAAACAAACGCGCTACCAAG	CTAACACAGGTGCCACAGAG	45.0	55.0	57.9	58.2	546-562 bp	1.0	83.7
trnP-UGG-psaJ_p3	AAAACAAACGCGCTACCAAG	AACAGACCCGCTAAAGAACC	45.0	50.0	57.9	57.8	580-596 bp	1.0	83.5
trnP-UGG-psaJ_p4	CAAAACAAACGCGCTACCAA	TCTCTATCAACAGACCCGCT	45.0	50.0	58.2	57.9	589-605 bp	1.0	83.2
trnP-UGG-psaJ_p5	TTGTACCCAAAACAAACGCG	TCTCTATCAACAGACCCGCT	45.0	50.0	57.8	57.9	596-612 bp	1.0	83.0