Hotspot

trnE-UUC-trnT-GGU

LSC · 31504-32048 · 545 bp

Back to Peperomia

Pi 0.0462

MarkerSeek score 78.20

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 545
variable_sites: 22
pi: 0.0462
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TCGTAGAACCTTACCCCCAG	ACGTCTTACCATGGCGTTAC	55.0	50.0	58.1	58.0	601-670 bp	1.0	85.6
trnE-UUC-trnT-GGU_p2	TCGTAGAACCTTACCCCCAG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.1	58.1	593-662 bp	1.0	85.1
trnE-UUC-trnT-GGU_p3	TCGTAGAACCTTACCCCCAG	TGGCGTTACTCTACCACTGA	55.0	50.0	58.1	58.1	590-659 bp	1.0	85.0
trnE-UUC-trnT-GGU_p4	GCTGCCTCCTTGAAAGAGAG	ACGTCTTACCATGGCGTTAC	55.0	50.0	58.3	58.0	565-634 bp	1.0	84.8
trnE-UUC-trnT-GGU_p5	TTCGTAGAACCTTACCCCCA	ACGTCTTACCATGGCGTTAC	50.0	50.0	57.7	58.0	602-671 bp	1.0	84.2