Hotspot

rpoB-trnC-GCA

LSC · 27121-28252 · 1132 bp

Back to Peperomia

Pi 0.039

MarkerSeek score 74.10

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1132
variable_sites: 44
pi: 0.039
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ATGAAGTTCTTCGGCCAACC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.5	58.1	1303-1379 bp	1.0	78.5
rpoB-trnC-GCA_p2	ATGAAGTTCTTCGGCCAACC	TGGGGAAAAAGGGATTTGCA	50.0	45.0	58.5	57.9	1291-1367 bp	1.0	78.3
rpoB-trnC-GCA_p3	ATGAAGTTCTTCGGCCAACC	TTGTTGATTAGGCGACACCC	50.0	50.0	58.5	58.2	1321-1397 bp	1.0	77.6
rpoB-trnC-GCA_p4	GCCAACCCCCAATCAATGAA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.7	58.1	1290-1366 bp	1.0	76.4
rpoB-trnC-GCA_p5	GCCAACCCCCAATCAATGAA	TGGGGAAAAAGGGATTTGCA	50.0	45.0	58.7	57.9	1278-1354 bp	1.0	76.2