Hotspot

trnS-GCU-trnG-UCC

LSC · 7715-8622 · 908 bp

Back to Peperomia

Pi 0.0472

MarkerSeek score 69.50

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 908
variable_sites: 33
pi: 0.0472
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	TCCTGGACGTGAGGACTAAA	CGCGAGGTTCCTTAGGAAAA	50.0	50.0	57.7	57.9	1484-1648 bp	1.0	83.5
trnS-GCU-trnG-UCC_p2	CGGAGAGAGAGGGATTCGAA	CGCGAGGTTCCTTAGGAAAA	55.0	50.0	58.3	57.9	1285-1445 bp	1.0	83.3
trnS-GCU-trnG-UCC_p3	TCCTGGACGTGAGGACTAAA	GTTTTCATTCGGCTCCTCCA	50.0	50.0	57.7	58.2	1745-1907 bp	1.0	82.9
trnS-GCU-trnG-UCC_p4	TCCTGGACGTGAGGACTAAA	TGTTTTCATTCGGCTCCTCC	50.0	50.0	57.7	58.2	1746-1908 bp	1.0	82.9
trnS-GCU-trnG-UCC_p5	CGGAGAGAGAGGGATTCGAA	GTTTTCATTCGGCTCCTCCA	55.0	50.0	58.3	58.2	1546-1704 bp	1.0	82.8