Hotspot

ndhF

Genome · 106023-108263 · 2241 bp

Back to Pentarhizidium

Pi 0.0424

MarkerSeek score 69.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: Genome
length_bp: 2241
variable_sites: 95
pi: 0.0424
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GAATTGGAGCTTCGCGAAAC	TCGACTCCCAACCAAAAGAA	50.0	45.0	58.1	57.0	2328 bp	1.0	74.2
ndhF_p2	GAATTGGAGCTTCGCGAAAC	TTCGACTCCCAACCAAAAGA	50.0	45.0	58.1	57.0	2329 bp	1.0	74.2
ndhF_p3	GAATTGGAGCTTCGCGAAAC	TCGACTCCCAACCAAAAGAAG	50.0	47.6	58.1	58.2	2328 bp	1.0	73.1
ndhF_p4	GAATTGGAGCTTCGCGAAAC	TTCGACTCCCAACCAAAAGAA	50.0	42.9	58.1	57.7	2329 bp	1.0	72.1
ndhF_p5	GAATTGGAGCTTCGCGAAAC	TTTCGACTCCCAACCAAAAGA	50.0	42.9	58.1	57.7	2330 bp	1.0	72.1