Hotspot

atpE-atpB

Genome · 50427-50431 · 5 bp

Back to Pentarhizidium

Pi 0.2

MarkerSeek score 74.00

Primer available yes

Label atpE-atpB

Feature Metadata

label_name: atpE-atpB
feature_type: igs
region: Genome
length_bp: 5
variable_sites: 1
pi: 0.2
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpE-atpB_p1	ACGCGAAGATTCAATGCCAT	TCTCCCCGAACAAGCTTTTT	45.0	45.0	58.6	57.6	110 bp	1.0	72.9
atpE-atpB_p2	ACGCGAAGATTCAATGCCAT	AATCTCCCCGAACAAGCTTT	45.0	45.0	58.6	57.4	112 bp	1.0	71.1
atpE-atpB_p3	ACGCGAAGATTCAATGCCAT	ATCTCCCCGAACAAGCTTTT	45.0	45.0	58.6	57.4	111 bp	1.0	71.1
atpE-atpB_p4	ACGCGAAGATTCAATGCCA	TCTCCCCGAACAAGCTTTTT	47.4	45.0	58.1	57.6	110 bp	1.0	68.7
atpE-atpB_p5	ACGCGAAGATTCAATGCCAT	CAATACCGACGAAGCCACTG	45.0	55.0	58.6	59.0	80 bp	1.0	68.1