Hotspot

trnC-GCA-trnG-GCC

Genome · 29795-30220 · 426 bp

Back to Pentarhizidium

Pi 0.042
MarkerSeek score 73.00
Primer available yes
Label trnC-GCA-trnG-GCC

Feature Metadata

label_name
trnC-GCA-trnG-GCC
feature_type
igs
region
Genome
length_bp
426
variable_sites
17
pi
0.042
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-trnG-GCC_p1 GATTTGCAGTCCTCCGTCTT GTTCGATTCCCGCTATCCG 50.0 57.9 57.9 58.2 465-486 bp 1.0 76.1
trnC-GCA-trnG-GCC_p2 GATTTGCAGTCCTCCGTCTT GGGTTCGATTCCCGCTATC 50.0 57.9 57.9 57.8 467-488 bp 1.0 75.9
trnC-GCA-trnG-GCC_p3 GATTTGCAGTCCTCCGTCTT GGTTCGATTCCCGCTATCC 50.0 57.9 57.9 57.8 466-487 bp 1.0 75.9
trnC-GCA-trnG-GCC_p4 TCCTCCGTCTTACCACTTGA GTTCGATTCCCGCTATCCG 50.0 57.9 57.7 58.2 456-477 bp 1.0 74.6
trnC-GCA-trnG-GCC_p5 AGTCCTCCGTCTTACCACTT GTTCGATTCCCGCTATCCG 50.0 57.9 57.7 58.2 458-479 bp 1.0 74.5