Hotspot

trnS-GCU-ycf12

Genome · 7958-8517 · 560 bp

Back to Pentarhizidium

Pi 0.06
MarkerSeek score 81.10
Primer available yes
Label trnS-GCU-ycf12

Feature Metadata

label_name
trnS-GCU-ycf12
feature_type
igs
region
Genome
length_bp
560
variable_sites
33
pi
0.06
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-ycf12_p1 GGGAGAGAGAGGGATTCGAA TGATGCAACTACCAATGCCA 55.0 45.0 57.9 57.8 692-702 bp 1.0 82.6
trnS-GCU-ycf12_p2 AAGGGAGAGAGAGGGATTCG TGATGCAACTACCAATGCCA 55.0 45.0 57.9 57.8 694-704 bp 1.0 82.6
trnS-GCU-ycf12_p3 AGCAATCCGACGCTTTAGAC TGATGCAACTACCAATGCCA 50.0 45.0 58.4 57.8 641-651 bp 1.0 80.8
trnS-GCU-ycf12_p4 GGGAGAGAGAGGGATTCGAA GGTCCTGATGCAACTACCAA 55.0 50.0 57.9 57.5 697-707 bp 1.0 80.4
trnS-GCU-ycf12_p5 AAGGGAGAGAGAGGGATTCG GGTCCTGATGCAACTACCAA 55.0 50.0 57.9 57.5 699-709 bp 1.0 80.3