Hotspot

ycf1

SSC · 121626-126716 · 5091 bp

Back to Pentanema

Pi 0.000524

MarkerSeek score 52.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5091
variable_sites: 4
pi: 0.000524
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GACGGTCCATTATCCTGAAGA	CGCAGAATCGCTCTACAACT	47.6	50.0	56.9	58.1	296 bp	1.0	63.1
ycf1_p2	AGACGGTCCATTATCCTGAAG	CGCAGAATCGCTCTACAACT	47.6	50.0	56.9	58.1	297 bp	1.0	63.0
ycf1_p3	AGACGGTCCATTATCCTGAAGA	CGCAGAATCGCTCTACAACT	45.5	50.0	58.4	58.1	297 bp	1.0	61.0
ycf1_p4	ACGGTCCATTATCCTGAAGA	CGCAGAATCGCTCTACAACT	45.0	50.0	55.6	58.1	295 bp	1.0	61.0
ycf1_p5	AGACGGTCCATTATCCTGAA	CGCAGAATCGCTCTACAACT	45.0	50.0	55.6	58.1	297 bp	1.0	61.0