Hotspot

clpP

LSC · 68403-70412 · 2010 bp

Back to Pentanema

Pi 0

MarkerSeek score 45.20

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2010
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ATTGACGCTAAATCCAGGCT	GGCTTTGTTACGTTTCCACA	45.0	45.0	57.3	56.9	2095-2096 bp	1.0	65.2
clpP_p2	ACTCGGCAGGGTTAATTTCA	GGCTTTGTTACGTTTCCACA	45.0	45.0	57.1	56.9	2123-2124 bp	1.0	64.0
clpP_p3	ATTGACGCTAAATCCAGGCT	AGGCTTTGTTACGTTTCCACA	45.0	42.9	57.3	58.4	2096-2097 bp	1.0	63.5
clpP_p4	AATTGACGCTAAATCCAGGCT	GGCTTTGTTACGTTTCCACA	42.9	45.0	58.0	56.9	2096-2097 bp	1.0	62.9
clpP_p5	ATTGACGCTAAATCCAGGCT	AGGCTTTGTTACGTTTCCAC	45.0	45.0	57.3	56.6	2096-2097 bp	1.0	62.8