Hotspot

trnL-ycf2

IRa · 141629-142047 · 419 bp

Back to Pentanema

Pi 0.0207

MarkerSeek score 47.80

Primer available yes

Label trnL-ycf2

Feature Metadata

label_name: trnL-ycf2
feature_type: igs
region: IRa
length_bp: 419
variable_sites: 13
pi: 0.0207
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-ycf2_p1	AAATGGTAGACACGCGAGAC	CCGGAAAGATATGTGGCCAT	50.0	50.0	58.0	57.7	510-994 bp	1.0	83.3
trnL-ycf2_p2	TGAAATGGTAGACACGCGAG	CCGGAAAGATATGTGGCCAT	50.0	50.0	58.0	57.7	512-996 bp	1.0	83.3
trnL-ycf2_p3	AAATGGTAGACACGCGAGAC	CCCATTCCTTAGCCGGAAAG	50.0	55.0	58.0	58.3	522-1006 bp	1.0	82.9
trnL-ycf2_p4	TGAAATGGTAGACACGCGAG	CCCATTCCTTAGCCGGAAAG	50.0	55.0	58.0	58.3	524-1008 bp	1.0	82.9
trnL-ycf2_p5	GCCTTGGTGGTGAAATGGTA	CCGGAAAGATATGTGGCCAT	50.0	50.0	58.1	57.7	522-1006 bp	1.0	82.7