Hotspot

ndhF-rpl32

SSC · 109898-110979 · 1082 bp

Back to Pentanema

Pi 0.000616

MarkerSeek score 46.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1082
variable_sites: 1
pi: 0.000616
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	CGCCCAATACCCTTTCCTTT	50.0	50.0	58.0	58.2	1261 bp	1.0	78.6
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	CGCCCAATACCCTTTCCTTT	45.0	50.0	58.0	58.2	1259 bp	1.0	78.5
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	CGCCCAATACCCTTTCCTTT	45.0	50.0	58.0	58.2	1260 bp	1.0	78.5
ndhF-rpl32_p4	GTTGCCGTCGGAAAAAGAAG	CTTTTAACGCCGCCCAATAC	50.0	50.0	58.0	57.8	1271 bp	1.0	78.3
ndhF-rpl32_p5	TGCCGTCGGAAAAAGAAGAA	CTTTTAACGCCGCCCAATAC	45.0	50.0	58.0	57.8	1269 bp	1.0	78.3