Hotspot

psaA-ycf3

LSC · 41062-41786 · 725 bp

Back to Pentanema

Pi 0.000921

MarkerSeek score 47.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 725
variable_sites: 1
pi: 0.000921
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	AGAAATGACCCGGTTTAGCC	CGACCAAGCTGCTGAGTATT	50.0	50.0	57.9	58.0	921-922 bp	1.0	79.1
psaA-ycf3_p2	AGAAATGACCCGGTTTAGCC	ACCAAGCTGCTGAGTATTGG	50.0	50.0	57.9	57.9	919-920 bp	1.0	78.2
psaA-ycf3_p3	CCCATGCCTCGAATGAAGTT	CGACCAAGCTGCTGAGTATT	50.0	50.0	58.2	58.0	903-904 bp	1.0	78.1
psaA-ycf3_p4	GTTTAGCCCATGCCTCGAAT	CGACCAAGCTGCTGAGTATT	50.0	50.0	58.3	58.0	909-910 bp	1.0	77.5
psaA-ycf3_p5	TTTAGCCCATGCCTCGAATG	CGACCAAGCTGCTGAGTATT	50.0	50.0	58.3	58.0	908-909 bp	1.0	77.5