Hotspot

trnG-UCC-psbD

LSC · 30697-32087 · 1391 bp

Back to Pentanema

Pi 0

MarkerSeek score 58.90

Primer available yes

Label trnG-UCC-psbD

Feature Metadata

label_name: trnG-UCC-psbD
feature_type: igs
region: LSC
length_bp: 1391
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-psbD_p1	AATCAAACCGAAAGACCCCT	GAAACGGTCCCTACGTAACC	45.0	55.0	57.0	58.0	1550-1552 bp	1.0	72.3
trnG-UCC-psbD_p2	ATCAAACCGAAAGACCCCTT	GAAACGGTCCCTACGTAACC	45.0	55.0	57.0	58.0	1549-1551 bp	1.0	72.3
trnG-UCC-psbD_p3	CAATCAAACCGAAAGACCCC	GAAACGGTCCCTACGTAACC	50.0	55.0	57.0	58.0	1551-1553 bp	1.0	72.0
trnG-UCC-psbD_p4	AATCAAACCGAAAGACCCCT	GGACCAGCCTACAAAAACGA	45.0	50.0	57.0	58.1	1568-1570 bp	1.0	71.4
trnG-UCC-psbD_p5	ATCAAACCGAAAGACCCCTT	GGACCAGCCTACAAAAACGA	45.0	50.0	57.0	58.1	1567-1569 bp	1.0	71.4