Hotspot

rpl32-trnL-UAG

SSC · 114783-115875 · 1093 bp

Back to Pentactina

Pi 0.0112

MarkerSeek score 66.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1093
variable_sites: 12
pi: 0.0112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGATATCGGGCAGCATTGAA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	1202-1212 bp	1.0	81.2
rpl32-trnL-UAG_p2	GGATATCGGGCAGCATTGAA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.1	58.0	1203-1213 bp	1.0	81.1
rpl32-trnL-UAG_p3	GGGCAGCATTGAAAGCTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.8	58.0	1195-1205 bp	1.0	80.6
rpl32-trnL-UAG_p4	GGGCAGCATTGAAAGCTTTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.8	58.0	1196-1206 bp	1.0	80.6
rpl32-trnL-UAG_p5	TCTTTCTACCGGGAGTTCGA	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.8	58.0	1161-1171 bp	1.0	80.4