Hotspot

ndhF-rpl32

SSC · 113554-114623 · 1070 bp

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Pi 0.0122
MarkerSeek score 65.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1070
variable_sites
13
pi
0.0122
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AAAGTAGAAGTCCTGCTCCA TTCAATGCTGCCCGATATCC 45.0 50.0 55.8 58.1 1244-1250 bp 1.0 62.7
ndhF-rpl32_p2 AAGTAGAAGTCCTGCTCCAA TTCAATGCTGCCCGATATCC 45.0 50.0 55.8 58.1 1243-1249 bp 1.0 62.7
ndhF-rpl32_p3 AAAGTAGAAGTCCTGCTCCA AAAAGCTTTCAATGCTGCCC 45.0 45.0 55.8 57.8 1251-1257 bp 1.0 62.2
ndhF-rpl32_p4 AAGTAGAAGTCCTGCTCCAA AAAAGCTTTCAATGCTGCCC 45.0 45.0 55.8 57.8 1250-1256 bp 1.0 62.2
ndhF-rpl32_p5 AAAGTAGAAGTCCTGCTCCAA TTCAATGCTGCCCGATATCC 42.9 50.0 56.6 58.1 1244-1250 bp 1.0 61.1