Hotspot

rpl22-rps19

LSC · 84777-84843 · 67 bp

Back to Pentactina

Pi 0.0448

MarkerSeek score 68.40

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 67
variable_sites: 3
pi: 0.0448
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TGTTGGCCTAAAGCGTATACT	TCTAAATTTCCGGGGGCATG	42.9	50.0	57.1	57.9	172 bp	1.0	65.7
rpl22-rps19_p2	TGGCCTAAAGCGTATACTTCTG	TCTAAATTTCCGGGGGCATG	45.5	50.0	57.9	57.9	169 bp	1.0	64.5
rpl22-rps19_p3	GGCCTAAAGCGTATACTTCTGT	TCTAAATTTCCGGGGGCATG	45.5	50.0	57.9	57.9	168 bp	1.0	64.5
rpl22-rps19_p4	TGTTGGCCTAAAGCGTATACT	CTCTAAATTTCCGGGGGCAT	42.9	50.0	57.1	57.6	173 bp	1.0	63.3
rpl22-rps19_p5	TGTTGGCCTAAAGCGTATACT	ACGGATCGTATGGTAGGACA	42.9	50.0	57.1	57.6	216 bp	1.0	63.1