Hotspot

rpl36-rps8

LSC · 80438-80912 · 475 bp

Back to Pentactina

Pi 0.0197

MarkerSeek score 66.80

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 475
variable_sites: 9
pi: 0.0197
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	ACTATAATTCGTCCCCGCCT	ATAGAAGGAATCGGCGGAGA	50.0	50.0	58.3	58.0	574-591 bp	1.0	81.3
rpl36-rps8_p2	ACTATAATTCGTCCCCGCCT	GAAGGAATCGGCGGAGAAAT	50.0	50.0	58.3	58.0	571-588 bp	1.0	80.9
rpl36-rps8_p3	GCCTACGGATCAATCGACAT	ATAGAAGGAATCGGCGGAGA	50.0	50.0	57.6	58.0	558-575 bp	1.0	80.1
rpl36-rps8_p4	GCCTACGGATCAATCGACAT	GAAGGAATCGGCGGAGAAAT	50.0	50.0	57.6	58.0	555-572 bp	1.0	79.8
rpl36-rps8_p5	ACTATAATTCGTCCCCGCCT	TAGAAGGAATCGGCGGAGAA	50.0	50.0	58.3	58.2	573-590 bp	1.0	79.5