Hotspot

rps11-rpl36

LSC · 80162-80323 · 162 bp

Back to Pentactina

Pi 0.0679
MarkerSeek score 70.70
Primer available yes
Label rps11-rpl36

Feature Metadata

label_name
rps11-rpl36
feature_type
igs
region
LSC
length_bp
162
variable_sites
11
pi
0.0679
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps11-rpl36_p1 CCAATTTTTGGTATAGGTTTTGCC AAGGGCCTCTGTTCGTAAAA 37.5 45.0 57.3 57.1 294 bp 1.0 43.0
rps11-rpl36_p2 CCAATTTTTGGTATAGGTTTTGCC AAATAAGGGCCTCTGTTCGT 37.5 45.0 57.3 56.8 298 bp 1.0 43.0
rps11-rpl36_p3 CCAATTTTTGGTATAGGTTTTGCC AGGGCCTCTGTTCGTAAAAT 37.5 45.0 57.3 56.8 293 bp 1.0 43.0
rps11-rpl36_p4 TTTTGGTATAGGTTTTGCCA AAGGGCCTCTGTTCGTAAAA 35.0 45.0 52.9 57.1 289 bp 1.0 43.0
rps11-rpl36_p5 TTTTTGGTATAGGTTTTGCCA AAGGGCCTCTGTTCGTAAAA 33.3 45.0 53.9 57.1 290 bp 1.0 43.0