Hotspot

rpl20-rps12

LSC · 70383-71186 · 804 bp

Back to Pentactina

Pi 0.0139

MarkerSeek score 66.20

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 804
variable_sites: 11
pi: 0.0139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTTGAATGAGCCCCTCGAAA	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	950-960 bp	1.0	76.5
rpl20-rps12_p2	TTGAATGAGCCCCTCGAAAG	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	949-959 bp	1.0	76.5
rpl20-rps12_p3	CTTGAATGAGCCCCTCGAAA	CAGCGACGAGGAACATGTAC	50.0	55.0	57.9	58.7	913-923 bp	1.0	75.1
rpl20-rps12_p4	TTGAATGAGCCCCTCGAAAG	CAGCGACGAGGAACATGTAC	50.0	55.0	57.9	58.7	912-922 bp	1.0	75.1
rpl20-rps12_p5	TTGAATGAGCCCCTCGAAAG	AGCGACGAGGAACATGTACT	50.0	50.0	57.9	58.8	911-921 bp	1.0	74.3