Hotspot

psaI-ycf4

LSC · 60997-61430 · 434 bp

Back to Pentactina

Pi 0.0189

MarkerSeek score 59.80

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 434
variable_sites: 8
pi: 0.0189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	AGTATTTCCGGCACTTGCAA	AGACCCCGCGATAAGTTCTA	45.0	50.0	58.1	57.9	536-546 bp	1.0	81.4
psaI-ycf4_p2	GCACTTGCAATGGCTTCTTT	AGACCCCGCGATAAGTTCTA	45.0	50.0	57.8	57.9	526-536 bp	1.0	80.9
psaI-ycf4_p3	AGTATTTCCGGCACTTGCAA	TACGTTCTGATCGCCAACTC	45.0	50.0	58.1	57.7	510-520 bp	1.0	79.8
psaI-ycf4_p4	GGCTTAGTATTTCCGGCACT	AGACCCCGCGATAAGTTCTA	50.0	50.0	57.7	57.9	541-551 bp	1.0	79.5
psaI-ycf4_p5	GCACTTGCAATGGCTTCTTT	TACGTTCTGATCGCCAACTC	45.0	50.0	57.8	57.7	500-510 bp	1.0	79.4