Hotspot

trnG-GCC-trnfM-CAU

LSC · 37879-38063 · 185 bp

Back to Pentactina

Pi 0.0282

MarkerSeek score 67.40

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 185
variable_sites: 5
pi: 0.0282
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	AGAAGATGCGGGTTCGATTC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	248-256 bp	1.0	83.4
trnG-GCC-trnfM-CAU_p2	GAGAAGATGCGGGTTCGATT	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	249-257 bp	1.0	83.3
trnG-GCC-trnfM-CAU_p3	ATGCGGATATGGTCGAATGG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.9	58.0	289-297 bp	1.0	82.5
trnG-GCC-trnfM-CAU_p4	AGAAGATGCGGGTTCGATTC	TGAGGTCACGGGTTCAAATC	50.0	50.0	58.1	57.8	246-254 bp	1.0	82.3
trnG-GCC-trnfM-CAU_p5	GAGAAGATGCGGGTTCGATT	TGAGGTCACGGGTTCAAATC	50.0	50.0	58.1	57.8	247-255 bp	1.0	82.2