Hotspot

trnS-UGA-psbZ

LSC · 37050-37419 · 370 bp

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Pi 0.00543

MarkerSeek score 54.80

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 370
variable_sites: 2
pi: 0.00543
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAAGACCGGAGCTATCAACC	CAACCCTCAGGAGAAGCAAA	55.0	50.0	58.1	57.7	504-508 bp	1.0	77.9
trnS-UGA-psbZ_p2	CAAGACCGGAGCTATCAACC	TTTTGTTACCCGACCAACCC	55.0	50.0	58.1	58.3	518-522 bp	1.0	77.7
trnS-UGA-psbZ_p3	CTATCAACCACTCGGCCATC	CAACCCTCAGGAGAAGCAAA	55.0	50.0	58.1	57.7	493-497 bp	1.0	77.4
trnS-UGA-psbZ_p4	CTATCAACCACTCGGCCATC	TTTTGTTACCCGACCAACCC	55.0	50.0	58.1	58.3	507-511 bp	1.0	77.1
trnS-UGA-psbZ_p5	AAGACCGGAGCTATCAACCA	CAACCCTCAGGAGAAGCAAA	50.0	50.0	58.4	57.7	503-507 bp	1.0	75.0