Hotspot

psbM-trnD-GUC

LSC · 30415-31639 · 1225 bp

Back to Pentactina

Pi 0.014

MarkerSeek score 60.20

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1225
variable_sites: 17
pi: 0.014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	GCCTTGGCTGACTGTTTTTAC	TTCAATTGGTCAGAGCACCG	47.6	50.0	58.0	58.5	1382-1388 bp	1.0	69.6
psbM-trnD-GUC_p2	CCTTGGCTGACTGTTTTTACG	TTCAATTGGTCAGAGCACCG	47.6	50.0	57.7	58.5	1381-1387 bp	1.0	67.5
psbM-trnD-GUC_p3	GCCTTGGCTGACTGTTTTTA	TTCAATTGGTCAGAGCACCG	45.0	50.0	56.5	58.5	1382-1388 bp	1.0	66.0
psbM-trnD-GUC_p4	GCCTTGGCTGACTGTTTTTAC	GTTCAATTGGTCAGAGCACC	47.6	50.0	58.0	57.0	1383-1389 bp	1.0	65.4
psbM-trnD-GUC_p5	CGCCTTGGCTGACTGTTTTT	TTCAATTGGTCAGAGCACCG	50.0	50.0	59.6	58.5	1383-1389 bp	1.0	64.8