Hotspot

petN-psbM

LSC · 29706-30303 · 598 bp

Back to Pentactina

Pi 0.0217

MarkerSeek score 56.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 598
variable_sites: 13
pi: 0.0217
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	AGTGTTTGGTGGGGTTTGTT	45.0	45.0	58.0	58.1	1173-1177 bp	1.0	81.0
petN-psbM_p2	TCTTGCTTGGGCTGCTTTAA	TGTACAAAGAGGGCGGTAGA	45.0	50.0	58.0	58.1	1102-1106 bp	1.0	81.0
petN-psbM_p3	TCTTGCTTGGGCTGCTTTAA	TGATTGTACAAAGAGGGCGG	45.0	50.0	58.0	57.9	1106-1110 bp	1.0	80.7
petN-psbM_p4	TCTTGCTTGGGCTGCTTTAA	GATTGTACAAAGAGGGCGGT	45.0	50.0	58.0	57.9	1105-1109 bp	1.0	80.7
petN-psbM_p5	ATCTTGCTTGGGCTGCTTTA	AGTGTTTGGTGGGGTTTGTT	45.0	45.0	57.8	58.1	1174-1178 bp	1.0	79.3