Hotspot

trnR-UCU-atpA

LSC · 10129-10726 · 598 bp

Back to Pentactina

Pi 0.058
MarkerSeek score 81.50
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
598
variable_sites
28
pi
0.058
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.81
markerseek_score
81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 TGGAATGAAAAGCGTCCATTG GAGGAAGCAGAAGCCCTTTT 42.9 50.0 57.4 58.1 672-756 bp 1.0 73.9
trnR-UCU-atpA_p2 GGAATGAAAAGCGTCCATTGT GAGGAAGCAGAAGCCCTTTT 42.9 50.0 57.4 58.1 671-755 bp 1.0 73.9
trnR-UCU-atpA_p3 TGGAATGAAAAGCGTCCATTG TTTACCGAGGAAGCAGAAGC 42.9 50.0 57.4 57.9 678-762 bp 1.0 73.8
trnR-UCU-atpA_p4 GGAATGAAAAGCGTCCATTGT TTTACCGAGGAAGCAGAAGC 42.9 50.0 57.4 57.9 677-761 bp 1.0 73.7
trnR-UCU-atpA_p5 GGTTCAAATCCTATTGGACGC GAGGAAGCAGAAGCCCTTTT 47.6 50.0 57.3 58.1 611-695 bp 1.0 73.2