Hotspot

trnS-GCU-trnG-UCC

LSC · 8500-9133 · 634 bp

Back to Pentactina

Pi 0.0119
MarkerSeek score 69.70
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
634
variable_sites
7
pi
0.0119
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ACCGAAAGATCCCTTAACTCT 50.0 42.9 58.0 56.1 698-739 bp 1.0 61.3
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ACCGAAAGATCCCTTAACTCT 50.0 42.9 58.0 56.1 697-738 bp 1.0 61.3
trnS-GCU-trnG-UCC_p3 ACGGAAAGAGAGGGATTCGA ACCGAAAGATCCCTTAACTCT 50.0 42.9 58.2 56.1 752-793 bp 1.0 60.1
trnS-GCU-trnG-UCC_p4 CAATCCGACGCTTTAGTCCA AACCGAAAGATCCCTTAACTCT 50.0 40.9 58.0 56.8 699-740 bp 1.0 59.3
trnS-GCU-trnG-UCC_p5 AATCCGACGCTTTAGTCCAC AACCGAAAGATCCCTTAACTCT 50.0 40.9 58.0 56.8 698-739 bp 1.0 59.3