Hotspot

ycf1

SSC · 128808-134135 · 5328 bp

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Pi 0.381

MarkerSeek score 76.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5328
variable_sites: 1833
pi: 0.381
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TACCATACTGAAACGGCTGC	ATCGTTCCATAGCGAATCGG	50.0	50.0	58.0	57.9	995 bp	0.5	59.3
ycf1_p2	ACTGAAACGGCTGCCATTAT	ATCGTTCCATAGCGAATCGG	45.0	50.0	57.9	57.9	989 bp	0.5	58.4
ycf1_p3	ATACTGAAACGGCTGCCATT	ATCGTTCCATAGCGAATCGG	45.0	50.0	57.9	57.9	991 bp	0.5	58.4
ycf1_p4	TACCATACTGAAACGGCTGC	GTTCCATAGCGAATCGGGAA	50.0	50.0	58.0	57.8	992 bp	0.5	58.0
ycf1_p5	GAAACGGCTGCCATTATTGG	ATCGTTCCATAGCGAATCGG	50.0	50.0	57.8	57.9	986 bp	0.5	57.8