Hotspot

rps15

SSC · 128147-128413 · 267 bp

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Pi 0.472

MarkerSeek score 77.40

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 267
variable_sites: 119
pi: 0.472
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	ACTCCCTCAACCCGTTCTAA	GCAGCCGTTTCAGTATGGTA	50.0	50.0	58.0	58.0	1437 bp	0.5	59.7
rps15_p2	CAATGCCTACTCCCTCAACC	GCAGCCGTTTCAGTATGGTA	55.0	50.0	58.0	58.0	1445 bp	0.5	59.5
rps15_p3	CCAACAATGCCTACTCCCTC	GCAGCCGTTTCAGTATGGTA	55.0	50.0	58.0	58.0	1449 bp	0.5	59.5
rps15_p4	TCCTAACTGTTCCGGTCCAT	GCAGCCGTTTCAGTATGGTA	50.0	50.0	58.1	58.0	1090 bp	0.5	59.4
rps15_p5	AACACCATGCATTGATGGGT	GCAGCCGTTTCAGTATGGTA	45.0	50.0	58.1	58.0	895 bp	0.5	59.4