Hotspot

ndhI

SSC · 123851-124336 · 486 bp

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Pi 0.467

MarkerSeek score 80.40

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 486
variable_sites: 209
pi: 0.467
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	CAAGAACCAGTCCCAACGAA	GAATTTGTGGCGTCAACCTG	50.0	50.0	58.0	57.9	1598 bp	0.5	59.3
ndhI_p2	TCCCAACGAAAAGGCAGAAA	GAATTTGTGGCGTCAACCTG	45.0	50.0	57.9	57.9	1588 bp	0.5	59.1
ndhI_p3	CAAGAACCAGTCCCAACGAA	ACAGTTCTTTACTTGGGCGG	50.0	50.0	58.0	58.1	1402 bp	0.5	58.8
ndhI_p4	CAAGAACCAGTCCCAACGAA	CAGTTCTTTACTTGGGCGGT	50.0	50.0	58.0	58.1	1401 bp	0.5	58.8
ndhI_p5	CCCAACGGTCCAAAGATGAA	GAATTTGTGGCGTCAACCTG	50.0	50.0	58.1	57.9	1756 bp	0.5	58.8